Establishment of a normal medakafish spermatogonial cell line capable of sperm production in vitro

Establishment of a normal medakafish spermatogonial cell line capable of sperm production in vitro.

Spermatogonia are the male germ stem cells that continuously produce sperm for the next generation. Spermatogenesis is a complicated process that proceeds through mitotic phase of stem cell renewal and differentiation, meiotic phase, and postmeiotic phase of spermiogenesis. Full recapitulation of spermatogenesis in vitro has been impossible, as generation of normal spermatogonial stem cell line...

Establishment of a pheasant (Phasianus colchicus) spermatogonial stem cell line for the production of interspecies germ line chimeras

Establishment of a human plasma cell line in vitro.

A human plasma cell line designated ARH-77 has been established and propagated in culture for the past 2 years. The cells exhibited morphological characteristics of plasma cells under light and electron microscopic examination. An average of 40% cells are positive for immunoglobulin G by direct immunofluorescence, while an immunoglobulin G-specific radioimmunoassay reveals the production of 1.2...

Production of medakafish chimeras from a stable embryonic stem cell line.

Embryonic stem (ES) cell lines provide a unique tool for introducing targeted or random genetic alterations through gene replacement, insertional mutagenesis, and gene addition because they offer the possibility for in vitro selection for the desired, but extremely rare, recombinant genotypes. So far only mouse blastocyst embryos are known to have the competence to give rise to such ES cell lin...

Establishment a CHO Cell Line Expressing Human CD52 Molecule

Background: CD52 is a small glycoprotein with a GPI anchor at its C-terminus. CD52 is expressed by Normal and malignant T and B lymphocytes and monocytes. There are detectable amounts of soluble CD52 in plasma of patients with CLL and could be used as a tumor marker. Although the biological function of CD52 is unknown but it seems that CD52 may be involved in migration and activation of T-cells...